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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: J Immunol. 2009 May 1;182(9):5446–5452. doi: 10.4049/jimmunol.0804052

Figure 6.

Figure 6

LAIR-1/Fc wt binds collagen type I with greater avidity than LAIR-1/Fc R65K. Rat collagen type I was covalently coupled to a biosensor surface as described in the Materials and Methods, and graded concentrations of LAIR-1/Fc wt (A) or LAIR-1/Fc R65K (B) in doubling dilutions from 410 nM to 3 nM were offered to the surface. Binding was initiated at t = 0 s and the washout (dissociation phase) was begun at t = 240 s. Experimental curves are indicated in the heavier lines marked with geometrical symbols and the curve fits to the data are indicated by the thinner solid lines. Binding isotherms for plateau values are plotted as a function of concentration;shown are a high density coupled (C) and low density coupled (D) surface respectively. Data for LAIR-1/Fc wt (solid circles), LAIR-1/Fc R65K (solid squares) and ICAM-1/Fc (solid circles with dashed line) are shown.