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Effect of culture and VCD on PND 4 ovaries. Ovaries were collected from PND 4 Fischer 344 rats on Day 0 (A) or cultured (8 days) in control medium (B) or medium containing 20 μM (C) or 30 μM (D) VCD. At the time of collection, ovaries were processed for histological evaluation as described in Materials and Methods. Small arrow, primordial follicles; thick arrow, primary follicles; arrowhead, secondary follicles. Bar = 25 μm; original magnification ×40.
