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. 2008 Apr 30;79(2):318–327. doi: 10.1095/biolreprod.108.067744

FIG. 8.

FIG. 8.

Effect of VCD on ovarian KIT and KITL protein. Postnatal Day 4 Fischer 344 rat ovaries were cultured (8 days) in control medium or medium containing 20 or 30 μM VCD. Total protein was extracted and analyzed as described in Materials and Methods. A) Densitometric analysis of KIT (120 + 145 kDa) protein expression on Day 8 in response to VCD (20 and 30 μM). B) Densitometric analysis of KITL protein expression on Day 8 in response to VCD (20 and 30 μM). Values represent the % of control; n = 3 (10 ovaries/pool); *P < 0.05, different from control. C) Representative Western blots of KIT, KITL, and ACTB. Lanes 1–3, control groups; lanes 4–6, VCD-exposed ovaries; lanes 7 and 8, KIT protein in adult rat ovary with or without blocking peptide.