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. 2008 May 14;79(2):367–375. doi: 10.1095/biolreprod.107.066357

FIG. 3.

FIG. 3.

Mitochondria integrity after 1 h of treatment in glucose-containing media. Sperm were stained with MitoTracker Red and mounted with Vectashield mounting medium with DAPI to examine mitochondria integrity. Sperm in the TL-BSA control and PCP 50-μM treatment groups showed strong and similar level of MitoTracker Red labeling in the midpieces, while sperm in the PCP 200-μM treatment group showed no MitoTracker Red labeling. In contrast, sperm in the ACH 0.5-mM treatment group showed intensive MitoTracker Red labeling, indicating that the mitochondria were very active.