FIG. 4.
Increasing mPOA Glu release and decreasing mPOA GABAA receptor activation with TPDC plus bicuculline rescues total LH release. Because middle-aged controls (M) and middle-aged controls with saline infusion at 0900 h and 1300 h (MS) were not significantly different, data from M are illustrated. Total LH (A), Glu (B), GABA (C), and Glu:GABA ratio (D) during the GnRH-LH surge in young control (Y; n = 8), M (n = 6), M infused at 0900 h and 1300 h with 10 μM bicuculline (M + B; n = 5), M reverse dialyzed with 10 mM TPDC (M + T; n = 6) and M treated with a combination of 10 μM bicuculline and 10 mM TPDC (M + B + T; n = 6) rats are illustrated. *Significantly different from Y (P < 0.01). **Significantly different from M (P < 0.05). AUC, Area under the curve.