FIG. 5.

UBE2I stimulates BrUTP incorporation independent of catalytic activity. A) Effect of UBE2I on BrUTP incorporation. Day 13 meiotically incompetent oocytes were injected with water (a, d, and g), wild-type Ube2i mRNA (b, e, and h), or Ube2i C93S mRNA (c, f, and i). The oocytes were then cultured for 18 h and then immunostained for UBE2I (a–c) and an anti-BrUTP antibody (d–f). g–i) Merged images of UBE2I and BrUTP staining. The experiment was performed three times, and shown are representative images. At least 50 oocytes were examined. The bars represent 10 μm. B) Quantification of BrUTP incorporation. For each experiment, the control was set to a value of 1, and the data (mean ± SEM) are expressed relative to the control, water-injected oocytes.