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. 2000 Sep 19;97(20):11074–11079. doi: 10.1073/pnas.190297597

Figure 2.

Figure 2

GSK3β inhibition prevents NGF- withdrawal-induced apoptosis in PC12 cells. (A) Photomicrographs of differentiated PC12 cells undergoing apoptosis as a result of NGF withdrawal (Top Left). Lithium (10 mM) pretreatment or addition of insulin (1 μM) prevented apoptosis and maintained relative intact cell morphology. (B) (Left) Effects of adenoviral-mediated transduction of mock (empty vector), wt GSK3β, or dominant negative K85R-GSK3β on apoptosis in PC12 cells. Apoptosis was measured by using the DNA fragmentation assay. K85R-GSK3β protected against NGF withdrawal-induced apoptosis. (Right) Photomicrograph of PC12 cells infected with K85R-GSK3β showing intact neurites (arrows) after withdrawal of NGF.