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Ischemia induces Y216 phosphorylation on GSK3β. (A) Western blot showing the increase in Y216 phosphorylation on GSK3β in the ipsilateral cortex (I) when compared with the contralateral cortex (C) at 3 and 6 h after MCAO. Total GSK3β in the tissue samples is shown in the lower Western blot. (B) Representative photomicrographs showing an increase in phosphorylated Y216 immunostaining in neurons in the ipsilateral (ipsi) vs. contralateral (contra) parietal cortex 6 h after MCAO.
