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. 2009 May 11;206(5):1047–1056. doi: 10.1084/jem.20082468

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© 2009 Robert et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Pharmacological inhibition of Parp activity results in increased CSR in CH12 cells. CH12 cells were stimulated with IL-4, TGF-β, and anti-CD40 antibody, in the presence or absence of 10 and 100 nM KU0058948 Parp inhibitor. After 3 d in culture, cells were analyzed by flow cytometry for cell surface expression of IgA in three independent experiments. Representative flow cytometry profiles are shown. The percentage of switched cells is indicated in each plot.