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. 2009 May 11;206(5):1057–1071. doi: 10.1084/jem.20082678

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© 2009 Shen et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — AFM of pKMP2 plasmids with nucleosomes. (A) Full-length plasmid (DNA contour length measured to be ∼1.1 µm or ∼3.2 kB). The magnification is 200,000×, with a 20-nm full-scale z axis. Using the AFM offline software, the full contour length of the free DNA was segmented into ∼50 linear segments, each ∼20 nm in length, and the segment lengths were tallied. (B) Portion of another pKMP2 plasmid with a single nucleosome. The magnification is 300,000×, with a 10-nm full-scale z axis. (C) The magnification is 300,000×, with a 10-nm full-scale z axis of a portion of the plasmid shown in A. (D) Single-line topographic AFM traces of the mononucleosome (left) and dinucleosome (right) as shown in B and C, respectively. The trace of the dinucleosome shows a bipartite topography and is roughly twice the size of the mononucleosome. The experiment was done twice with similar results.