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. 2009 May 11;206(5):1057–1071. doi: 10.1084/jem.20082678

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© 2009 Shen et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — Sequences of pKMP2 plasmids treated with AID during transcription and amplified in E. coli in the presence of ampicillin. The WT sequence is shown on the top lines. The two MP2 nucleosome positioning sequences are in green (BamHI sites underlined). The in-frame ACG initiation codons are in italics and underlined. The in-frame stop codons are underlined and not italicized. The AID-created Ts resulting in a potential TATA box present in all 35 clones are in bold font. The Shine-Dalgarno sequence (AGGAAG) is in blue. The transcription start site of unmutated pKMP2 plasmids in E. coli is in purple and underlined. The 5′ end of the Amp^r coding region is in red. The ACG start codon for Amp^r is at 3041–3043. Dashed lines represent agreement with the unmutated pKMP2 with mutations indicated by lowercase font. The sequences were obtained from one of three experiments.