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. 2009 Jun 8;206(6):1339–1350. doi: 10.1084/jem.20082316

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© 2009 Fukui et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — D34A mutation does not influence the subcellular distribution of Unc93B1. (A and B) WT, D34A, or H412R Unc93B1-GFP was expressed in DCs from 3d mice. Their subcellular distribution was determined by confocal microscopy (left). Cells were also stained with a marker locating (A) ER or (B) lysosome (center). (right) Merged images. (C) WT Unc93B1-GFP (left) and D34A Unc93B1-cherry (center) were coexpressed in DCs from 3d mice. These cells were left unstimulated or stimulated with 5 µg/ml imiquimod, 1 µM CpG-B, or 1 µg/ml lipid A for 3 h, as indicated in the figure. Green and red images were merged (right). These experiments were repeated three times and the represented images are shown. Bars, 5 µm.