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. 2009 Jun 8;206(6):1327–1337. doi: 10.1084/jem.20082173

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© 2009 Kuang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Expression pattern of PD-L1 was correlated with the activation pattern of monocytes/Mφ in peritumoral stroma. (A–C) FACS analysis of PD-L1 and HLA-DR expression in fresh monocytes isolated from peripheral blood and tissues. Representative data on PD-L1⁺HLA-DR^high monocytes (A), percentages of HLA-DR^highCD14^high monocytes (B), and the MFI of HLA-DR in HLA-DR^highCD14^high monocytes (C) are shown. The samples collected and the numbers of donors in A–C were the same as in Fig. 1. The data shown in A are representative dot plots of at least seven individuals from more than five independent experiments; B and C show the statistics analysis of these samples. The continuous and dashed horizontal bars in B represent median values. Results are expressed as means ± SEM. (D) Positive correlations between the levels of PD-L1⁺ and HLA-DR^high monocytes. The samples used in D were blood from healthy individuals, HCC patients, and paired nontumor and tumor tissues from HCC patients (n = 28 for each). (E) Adjacent sections of paraffin-embedded hepatoma samples stained with the indicated markers. The micrographs at higher magnification show the peritumoral stroma region (1) and a cancer nest (2). Significant difference compared with healthy blood is indicated (***, P < 0.0001). 1 out of 15 representative micrographs is shown in E. Bars, 150 µm.