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. 2009 Jun 8;206(6):1291–1301. doi: 10.1084/jem.20082767

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© 2009 Minegishi et al.

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Figure 2. — Supernatants of activated HIES T cells fail to stimulate keratinocytes to secrete significant amounts of antibacterial factors. In the presence or absence of anti–IL-17A plus anti–IL-22 (A and B), anti-BD3 (C), or isotype-matched control antibodies, primary human keratinocytes were incubated for 48 h with the supernatants of HIES (Pt.) or control (Cont.) T cells that had been stimulated (+) or not (−) with anti-CD3 and anti-CD28 for 72 h as in Fig. 1. (A) The concentration of CXCL8, BD2, and BD3 in keratinocytes supernatants was determined by ELISA. Representative data from one patient and one control are shown (mean ± SD; n = 3), and similar results were obtained from the other patients and controls. (B and C) The culture supernatants of keratinocytes were analyzed for their antistaphylococcal activity by the colony assay (mean ± SD; n = 3). The results shown in are representative of at least three independent experiments. **, P < 0.01.