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. 2009 Jun 8;206(6):1291–1301. doi: 10.1084/jem.20082767

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© 2009 Minegishi et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Keratinocytes and bronchial epithelial cells show greater dependence on Th17 cytokines for the production of chemokines and BDs than other cell types. Primary human keratinocytes, bronchial epithelial cells, dermal fibroblasts, endothelial cells (HUVEC and HMVEC-L), and macrophages were incubated for 48 h with T cell supernatants that were prepared as described in Fig. 1 A or with the Th17 cytokine cocktail (Th17 mix: IL-17A + IL-17F + IL-22), the classical proinflammatory cytokine cocktail (classical mix: TNF-α + IL-1β + IFN-γ), or the combination of both (both mix; B). The concentration of CXCL8, BD2, and BD3 in their culture supernatants was determined by ELISA. Representative data from one patient and one control are shown in A (mean ± SD; n = 3), and similar results were obtained from the other patients and controls. The results shown are representative of at least three independent experiments. **, P < 0.01.