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. 2009 Jun 8;206(6):1291–1301. doi: 10.1084/jem.20082767

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© 2009 Minegishi et al.

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Figure 5. — HIES T cells show poor ability of stimulating keratinocytes in response to staphylococcal superantigens and candida antigens. (A) PBMCs from HIES patients (Pt.) and control subjects (Cont.; n = 6 each, indicated by dots) were stimulated or not (−) with SEB (S, 100 ng/ml) or CAA (C, 1/20,000 vol/vol) for 5 d, and the concentration of the indicated cytokines in their culture supernatant was determined by ELISA. (B and C) Fibroblasts and keratinocytes were cultured for 48 h in the absence (−) or presence (S) of SEB or with the supernatants of patients (Pt.) or control (Cont.) PBMCs that had been unstimulated (−) or stimulated with SEB (S) as in A, in the presence or absence of anti–IL-17A + anti–IL-22 or isotype-matched control antibodies. Their culture supernatants were analyzed by ELISA for the secretion of CXCL8 and BD2 (B) and evaluated for their neutrophil chemotactic activity (C). The results shown are representative of two independent experiments. Error bars show mean ± SD (n = 3). **, P < 0.01.