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. 2009 Jun 8;206(6):1303–1316. doi: 10.1084/jem.20090299

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© 2009 Pöllinger et al.

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Figure 1. — Characterization of a new MOG-specific TCR transgenic SJL/J mouse. (A) Thymocytes from 8–10-wk-old TCR transgenic mice or NTLs were stained with antibodies to CD4, CD8, TCR-Vβ4, and TCR-Vα8.3 and cells were analyzed by flow cytometry. Representative figures of three to seven analyzed mice are shown. Transgenic Vα8.3 and Vβ4 are shown on cells gated as indicated, either CD4⁺/CD8⁻ or CD4⁻/ CD8⁺. (B) Proliferative response to recombinant MOG protein (rMOG) or MOG peptide 92–106 measured as ³H-thymidine incorporation. Splenocytes from TCR transgenic mice (8–10-wk-old healthy) were cultured with indicated concentrations of rMOG (top) and MOG_92-106 (bottom). (C) Cytokine responses to rMOG. Indicated cytokines were measured in supernatants harvested 48 h after rMOG activation by ELISA. Pooled data from three independent experiments are shown. Error bars indicate SEM. B and C: TCR¹⁶⁴⁰, n = 3; TCR¹⁶³⁹, n = 3; TCR¹⁵⁸⁶, n = 3; NTL, n = 2.