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. 2009 Jun 8;206(6):1303–1316. doi: 10.1084/jem.20090299

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© 2009 Pöllinger et al.

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Figure 7. — MOG-specific autoantibodies bind MOG-expressing cells and activate complement. (A) Binding of anti-MOG Ig from indicated mice to correctly conformed MOG on the cell surface of transduced EL4 cells (EL4-MOG) shown by flow cytometry. EL4 cells (top) and MOG-expressing EL4-MOG cells (bottom) were incubated with 1/200 diluted sera obtained from the indicated mice or 0.5 µg/ml 8.18-C5 mAb. Bound antibodies were detected by FACS using biotinylated anti–IgG1-specific antibody (allotype unspecific) and streptavidin-PE (SA-PE). A representative plot of three independent experiments is shown. (B) Complement activating capability of MOG binding antibodies in sera from transgenic mice. EL4 and EL4-MOG cells were incubated with sera (1/20 and 1/200 diluted) obtained from the indicated mice, each with three to five per group. Complement activating capability was measured by cell lysis using propidium iodide (PI) staining after incubation of sera-bound EL4 cells with rabbit complement. Background values were subtracted, and shown are mean values with the SEM of one experiment representative of three similar experiments.