(A) Six groups of mice were sensitized at days 0 and 14, and were challenged at days 28–38. (B, C) Total BALF cells and leukocytes in BALF were counted using HEMAVET HV950FS (Drew Scientific Inc.) at day 40. The eosinophils count (C) was re-displayed with small scale (* p<0.05, **p<0.01, ***p<0.001, significantly different from the Sham value, n = 4–6). (D) The IL-5 level in BALF was analyzed by mouse IL-5 ELISA kit (PIERCE) (* p<0.05, *** p<0.001, significantly different from the Sham value, n = 4–6). (E, F) Lung tissue (E) and nasal mucosa section (F) were prepared at day 40, and stained with periodic acid-Schiff (PAS) reagent. Mucus containing goblet cells were showed as deep purple. Scale bars indicate 50 µm.