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. 2009 Aug 3;4(8):e6490. doi: 10.1371/journal.pone.0006490

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Glaser et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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To determine the effect of NaCl and KGlu on the interaction strength, binding curves were generated for the interaction of either F-σ70 or F-σ32 with 10 nM Tb-core at 100 mM, 250 mM, or 500 mM NaCl or KGlu. Shown is the average acceptor/donor signal (A₅₂₀/A₄₉₀) for each sample. The error bars represent the standard deviation for a sample size of 4. The curves were fit with Origin 7 and the K_D values are listed for each curve. A) The effect of NaCl on the interaction of F-σ70 with 10 nM Tb-core. B) The effect of KGlu on the interaction of F-σ70 with 10 nM Tb-core. C) The effect of NaCl on the interaction of F-σ32 with 10 nM Tb-core. D) The effect of KGlu on the interaction of F-σ32 with 10 nM Tb-core.