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. 2009 Aug 3;4(8):e6480. doi: 10.1371/journal.pone.0006480

Figure 11. Testing the superposition hypothesis in muscle at long lengths.

Figure 11

A: A sequence of two elongation steps (0.5 mm each), separated in time (ISI) by 45 s, was applied, and the resulting force measured. B: The same two steps shown in A are applied, but now the ISI is only 10 ms. (Blue: Force measured. Red: Force predicted by the superposition principle) C: Same as in B, but with an ISI of 100 ms. D: Same as in C, but with an ISI of 1 s. For clarity, the maximum force recorded is marked by a small horizontal blue bar just to the left of the value. In all cases the prediction is initially considerably higher than the actual force, indicating that the superposition principle does not hold in muscle at long lengths.