Figure 4.

Role of IFNGR and STAT1 during in vitro Th1 differentiation and pS727-STAT1 involvement in the IS. (a) CD4⁺CD62L^highCD25⁻ Thps from wt or ifngr1^−/− mice were purified by magnetic bead separation, followed by flow cytometric sorting. Plate-bound anti-CD3 and anti-CD28 were used to activate cells, and after 5 d in culture the production of cytokines was evaluated by ICS and flow cytometry. Shown are results representative of six independent experiments. (b) Cells were sorted by negative selection of CD4⁺ T cells, activated by cross-linking of their TCRs, fixed, and stained as indicated, and images were acquired and treated (deconvolution) as in Fig. 1 (of four experiments; n = 73–83). Bars, 2 µm.