Figure 6.

IL4R is recruited to the IS in the absence of IFNGR1 or STAT1. Thps were isolated by negative magnetic separation from the LNs of young animals of different genetic backgrounds (B6, ifngr1^−/−, 129, and stat1^−/−). CD4⁺CD62L^high T cells (98% pure) were activated, fixed, and stained as indicated, and then imaged as described in Fig. 1. (a) Confocal images. Bars, 4 µm. (b) Quantification by linearization of whole-cell surface. Four independent experiments were analyzed, and the mean of ρ was calculated for each cell (n = 88–114), as in Fig. 2.