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IL4R is recruited to the IS in the absence of NFATc2 and NFATc3. Thps were isolated prepared as in Fig. 1 from the LNs of young WT or NFATc2c3 double-deficient mice. Thps were activated, fixed, and stained as indicated, and imaged as described in Fig. 1. (a) Confocal images. Bars, 4 µm. (b) Quantification by linearization of whole-cell surface. Two independent experiments were analyzed and the mean of ρ was calculated for each cell (n = 93–145), as in Fig. 2.
