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. Author manuscript; available in PMC: 2009 Jul 24.
Published in final edited form as: Dev Biol. 2006 Nov 22;303(2):501–513. doi: 10.1016/j.ydbio.2006.11.030

Fig. 3.

Fig. 3

(A) (a, b) Whole mount staining for alkaline phosphatase in rapamycin-treated embryos demonstrated normal blood vessel morphogenesis; (c, d) alcian blue staining demonstrated that branchial arch formation was not significantly affected by rapamycin, though the size of the cranium and jaw was slightly smaller in treated embryos. Numerals denote the branchial arches; (e, f) Immunofluorescence staining for α6F subunit of Na/K ATPase reveal that the pronephric ducts are structurally normal in rapamycin-treated larvae. (B) Sagital sections of 9 dpf larvae stained with H&E, control and treated with rapamycin, demonstrating the disproportionate and enduring effect of rapamycin on gut development relative to the rest of the embryo. Abbreviations: BA, branchial arches; SB, swim bladder; isv, intersomitic vessels; siv, sub-intestinal vessels; pd, pronephric duct; L, liver; g, gut; y, yolk. Original magnification: A, 40x; B, 100x.