Table 1.
Morpholino and RT-PCR oligonucleotide sequences
| Gene | Accession # | Morpholino sequence | Primers sequence (5′-3′) | (bp) |
|---|---|---|---|---|
| S6 kinase | NM_212786 | 296-EI
cagtcttcaacttacgtgaacaaga Start gtccggttcctcttcgcaaacattc |
Forward- GATGACTCCATGATTGAGGA
Reverse- CAGTCCATGTAAATGGTCTA |
470 |
| ztor | BC091880 | 2270-EI
ggtttgacacattaccctgagcatg 2221-IE cgctgaatactgctcaaaagaaaca |
Forward -GAGTTCATGTTCCTGCTG
Reverse- CCATCCAATGTAGCATTGG |
1200 |
| raptor | XM_684221 | EI-2
atggatggatggatgctcacctatc Start gttttttcctctgcgtctaccattg |
Forward -ACAGTGAGTGTGGCTCTAG
Reverse- CCT CCT TGG CAT TTC TGC GTA G |
270 |
| rictor | XM_685234 | EI-2
agtgcaataaaacgtggtacagacc Start tcggatgctgaccgccatctttacc |
Forward- GTGGCCAAACAACAAGGAGT
Reverse- CCTGATGAGGTAGCGGAGAG |
203 |