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. Author manuscript; available in PMC: 2009 Jul 24.
Published in final edited form as: J Biol Chem. 2006 Mar 22;281(19):13628–13635. doi: 10.1074/jbc.M513730200

Fig. 5.

Fig. 5

Glycerol gradient centrifugation. Cellular extracts from revertants 6H2 (A, B) and 6H3 (C, D) were fractionated by glycerol gradient centrifugation. Fractions were analyzed by Western blotting with a mouse antibody to the N-terminus of β-tubulin (A, C) or a rat antibody to one of the subunits of the CCT complex (B, D). Arrows indicate the migration of thryroglobulin (669 kDa), ferritin (440 kDa), and catalase (232 kDa). Labeled bands include wild-type β-tubulin (β), 6H2 mutant β-tubulin (6H2), 6H3 mutant β-tubulin (6H3), and CCT subunit TCP-1α (CCT). Note that the fractions containing the mutant subunits (14 in panel A, 12 in panel C) are also the fractions that stain heavily for CCT.