Table 2.

Biological and Medical Evaluation of Nanoparticle

Subjects of evaluation	Aspects of evaluation
Biodistribution	Whole organism, tissue and organ level
Localization	Different intracellular vesicle, organelles, cell level
Metabolic routes	Absorption, distribution, metabolism and excretion (ADME)
Immunological properties	IgG/IgM specific Abs, cytokine induction, T cell activation
In vivo degradation	Enzyme digestion, lysosomal decomposition
Biocompatibility	Biological environment and adverse health effect in vivo
Toxicological characters	Chemical composition, particle size, reactivity, structure/ properties, surface coating modification
Chemotherapeutic concerns	Therapeutic index of nanomedicines and their delivery systems relates with clinical administration
Etc ··········	Other unclear consequence associated with nanomedicines