. Author manuscript; available in PMC: 2010 Aug 15.

Published in final edited form as: J Immunol Methods. 2009 Jun 9;347(1-2):36–45. doi: 10.1016/j.jim.2009.05.011

Table 2.

CD4 T-cell cytokine expression in response to whole sucrose-purified UV-killed vaccinia and inhibition by blocking locus-specific anti-HLA class II antibodies.

		cytokine
subject	mAb	IFN-γ	IL-2	TNF-α
1 %(+) CD4^a		0.083	0.190	0.270
	anti-DR^b	75.9	72.1	77.0
	anti-DP^b	30.1	36.8	37.0
	anti-DQ^b	38.6	0.0	3.7
12 %(+) CD4		0.430	0.750	1.120
	anti-DR	85.3	82.7	79.5
	anti-DP	27.9	8.0	19.6
	anti-DQ	11.6	0.0	5.4
9 %(+) CD4		0.137	0.250	0.473
	anti-DR	72.6	78.2	89.5
	anti-DP	15.9	2.0	2.8
	anti-DQ	15.9	0.0	0.0
3 %(+) CD4		0.295	0.545	0.510
	anti-DR	66.1	67.0	62.7
	anti-DP	25.4	39.4	21.6
	anti-DQ	8.5	28.4	7.8
mean ± SD, 4 subjects	anti-DR	75.0 ± 8.0	75.0 ± 6.9	77.2 ± 11.1
	anti-DP	24.8 ± 6.3	21.6 ± 19.3	20.3 ± 14
	anti-DQ	18.6 ± 13.6	7.1 ± 14.2	4.2 ± 3.3

Percentage of lymphocyte forward/side scatter, live, CD3(+), CD4 (+) cells with specific accumulation of the indicated cytokine in response to vaccinia after subtraction of values for mock stimulation.

Percentage inhibition of the number of similarly gated cells by the indicated mAb.