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. Author manuscript; available in PMC: 2009 Jul 24.
Published in final edited form as: Mol Cancer Ther. 2008 May;7(5):1156–1163. doi: 10.1158/1535-7163.MCT-07-2183

Figure 6. Elevated Akt activity contributes to stabilization of Mcl-1L and c-FLIPL.

Figure 6

A, Phosphorylated-Akt (p-Akt) and total Akt in H460 and H460-TR cells were measured by Western blot. β-actin was detected as an input control. B, H460-TR cells were treated with 10 μM LY294002 overnight. Mcl-1L, c-FLIPL, and p-Akt were detected by Western blot. β-actin was detected as an input control. C, Pooled siRNA against Akt1, Akt2, and Akt3 was used to knock down Akt in H460-TR cells. p-Akt, total Akt, Mcl-1L, and c-FLIPL were measured by Western blot. D, Nontransfected, mock, and negative siRNA-transfected cells are shown as in H460-TR cells. Control cells were treated with 80 ng/mL TRAIL for 24 h and cell death was detected by LDH assay. Columns, mean of three experiments; bars, SD.