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. 2009 Jan-Feb;1(1):71–85. doi: 10.4161/mabs.1.1.7492

Figure 2.

Figure 2

Antibody secretion. (A) A total of 2 µg of purified protein (lanes 1, 3 and 5) and supernatant from transfected CHO-S cells (lanes 2, 4 and 6) were loaded onto a 12% SDS-PAGE gel and subjected to electrophoresis under non-reducing conditions. Wild type ImmunoBody™ antibody is shown in lanes 1 and 2, TRP2 grafted into the CDRH2 site alongside the gp100 210M CTL in CDRH1 and HepB help CD4 epitope in CDRL1 in lanes 3 and 4 and the TRP2 CTL epitope in CDRH3 in lanes 5 and 6. The nitrocellulose blot was incubated with a HRP goat anti human IgG Fc specific antibody. (B) Western blot analysis was carried out as above however the nitrocellulose blot was incubated with a HRP anti human kappa light chain antibody. (C) Hydropathicity plots of CDRH3 within the wild type ImmunoBody™ heavy chain and those incorporating CTL (TRP2) and CD4 (HepB help) epitopes. The Kyte and Doolitle70 hydropathicity index was utilized to calculate the hydropathicity distribution.