Table 4.

ImmunoBody™ protein secretion

DCIB			DNA construct					IB expression
no.	H1 site	H2 site	H3 site	L1 site	L2 site	L3 site	H chain*	L chain¥	Intact IB§
IB14	wt	wt	wt	wt	wt	wt	+++	+++	+++
IB78	wt	TRP2	wt	wt	wt	wt	+++	+++	−
IB31	wt	wt	TRP2	wt	wt	wt	+++	++	++
IB75	wt	wt	TRP2AAATRP2	wt	wt	wt	++	+++	−
IB36	wt	wt	wt	wt	wt	TRP2	+++	+	+
IB80	wt	wt	wt	wt	wt	TRP2AAATRP2	+++	−	−
IB49	wt	wt	HepB	wt	wt	wt	+++	+++	+++
IB79	wt	wt	wt	wt	wt	HepB	+++	−	−
IB18	wt	TRP2	wt	HepB	wt	wt	+++	−	−
IB32	wt	wt	TRP2	wt	wt	HepB	+++	−	−
IB82	wt	wt	HepBAAAHepB	wt	wt	wt	+++	+++	+++
IB76	wt	wt	HepB	wt	wt	TRP2	+++	+	+
IB15	gp100 210M	TRP2	wt	HepB	wt	wt	+++	−	−

+++ High expression; ++ Medium expression; + Low expression.

^*Heavy chain secretion was detected using a sandwich Elisa specific for heavy chain only.

^¥Light chain secretion was detected using a sandwich Elisa specific for light chain only.

^§Intact ImmunoBody™ secretion was measured using a heavy chain specific capture anti-serum and a light chain specific detection antibody.