Figure 1.

EBS configuration in the Stromelysin-1 promoter: differential binding stoichiometry and transactivation properties of p51 and p42 Ets-1 isoforms. (A) Schematic organization of the human Ets-1 p51 and p42 isoforms. DBD, DNA-binding domain; PNTD, pointed domain; ID, inhibitory domain. (B, C) Gel shift assays. Equal amounts of p51 (B) or p42 (C) were incubated with M1, M2, WT, IP and WT + 4 variants, as defined in Table 1. Binary (2) and ternary (3) complexes are indicated. Non-relevant complexes (see ‘Materials and Methods’ section) are indicated by an asterisk. (D) Transient transfection assays with reporter plasmids containing WT or variant EBS defined in Table 1 and either p51 or p42 eukaryotic expression vector. Luciferase activity was normalized and expressed as relative luciferase activity (RLU).