Table 3.

Spacer elongation in WT restrained simulations

Δda (Å)	ΔEb (Kcal/mol)	Icc (°)	Twavd(°)	Protein–protein steric clashes
0	0	5	37	++
2.5	4	22	34	+
4.5	15	42	30	∼0
7.5	37	67	23	–
8.5	44	82	20	–

^aΔd: increase of the distance separating two EBS core motifs in reference to the starting structure.

^bΔE: difference between total energies of constained and starting structures.

^cIc: curvative intensity.

^dTw_av: average twist of the 4 bp spacer.