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. 2009 May 12;37(13):4205–4217. doi: 10.1093/nar/gkp341

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Binding of Cwc2 to snRNAs in vitro. (A) SDS–PAGE analysis of purified, His-tagged, Cwc2 (lane 2). Molecular weight markers (lane 1) are indicated to the left of the panel in KiloDaltons. (B) EMSA using ³²P end-labeled RNAs that are indicated below the panel. RNA was incubated without (−) and with (+) 4.5 µM Cwc2-His, then complexes were resolved on 5% nondenaturing polyacrylamide gels. (C) Increasing concentrations of Cwc2-His protein were incubated with fixed amounts of the individual snRNAs indicated below the panels. Concentrations of Cwc2-His are indicated above each panel. Complexes were resolved on 5% nondenaturing polyacrylamide gels.