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. 2009 May 12;37(13):4205–4217. doi: 10.1093/nar/gkp341

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Binding of Cwc2 to the U4 and U6 snRNAs and different pre-mRNAs in vitro. (A) EMSA using ³²P end-labeled U4 and U6 snRNAs. Increasing concentrations of Cwc2-His protein were incubated with a fixed amount of a labeled RNA. The identity of the RNA is indicated below the panels. Concentrations of Cwc2-His are indicated above each panel. Complexes were resolved on 5% nondenaturing polyacrylamide gels. (B) Binding of Cwc2 to the ACT1, CYH2 and RPS10B pre-mRNAs in vitro. EMSA using ³²P end-labeled pre-mRNAs. Increasing concentrations of Cwc2-His protein were incubated with a fixed amount of a labeled RNA. The identity of the RNA is indicated below the panels. Concentrations of Cwc2-His are indicated above each panel. Complexes were resolved on 5% nondenaturing polyacrylamide gels.