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. 2009 May 12;37(13):4205–4217. doi: 10.1093/nar/gkp341

Figure 7.

Figure 7.

Binding of Cwc2 RRM fragment to snRNAs in vitro. (A) SDS–PAGE analysis of purified, His-tagged, Cwc2 RRM fragment (lane 2). Molecular weight markers (lane 1) are indicated to the left of the panel in KiloDaltons. (B) EMSA using 32P end-labeled RNAs. The identity of the RNA is indicated below the panel. RNA was incubated without (−) and with (+) 4.5 µM Cwc2-His RRM fragment, then complexes were resolved on 5% nondenaturing polyacrylamide gels.