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. 2009 May 19;37(13):4322–4330. doi: 10.1093/nar/gkp349

Figure 4.

Figure 4.

Localization and methylation of chimeric Npl3 proteins. (A) Chimeric Npl3 proteins are predominantly nuclear in the absence of HMT1. PSY865 (hmt1Δ) cells bearing either pPS1872 (+Hmt1) or pRS315 (−Hmt1) were transformed with plasmids expressing GFP-tagged chimeric Npl3 proteins. Localization of GFP-Npl3 was visualized after induction of expression for 1.5 h in mid-log-phase cells grown at 30°C. (B) Chimeric Npl3 proteins are methylated. Aliquots of the cultures shown in (A) were lysed in RIPA buffer, then proteins from these whole cell lysates were resolved by SDS-10% PAGE and analyzed by immunoblotting with monoclonal antibodies. Gels used for anti-GFP immunoblots contained 4 µg total protein in each lane; gels used for anti-dimethylarginine immunoblots contained 40 µg total protein in each lane.