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. 2009 May 22;37(13):4393–4406. doi: 10.1093/nar/gkp398

Figure 1.

Figure 1.

TGF-β blocks the repression of COL1A2 transcription by IFN-γ in HASMCs. (A, B) HASMCs were treated with IFN-γ and/or TGF-β for various time points as indicated. Collagen type I message levels were measured by real-time PCR. Data are expressed as relative RNA levels compared to control levels, normalized to 18S RNA and presented as average ± SD. (C) HASMCs were treated with IFN-γ and/or TGF-β, and measured for collagen protein levels by Western blot. A representative picture is shown. Normalized collagen levels were compared to the control group and expressed as relative densitometric values. Shown in the graph are data collected from three independent experiments and expressed as average ± SD. (D) HASMCs were transfected with different collagen type I promoter constructs along with GFP for normalization. Cells were treated with IFN-γ and/or TGF-β 24 h after transfection. Luciferase activities were assayed 48 h after transfection, normalized by both protein concentration and GFP fluorescence, and presented as average ± SD.