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. 2009 May 22;37(13):4393–4406. doi: 10.1093/nar/gkp398

Figure 3.

Figure 3.

RFXB alleviates the antagonism of IFN-γ mediated collagen transcription repression by TGF-β. (A) A collagen type I promoter construct (pH 20) was co-transfected with expression constructs for RFXB and/or RFXBSV into HASMCs. IFN-γ was added 24 h after transfection and incubated with cells for additional 24 h. Luciferase activities were normalized by both protein concentration and GFP fluorescence, and presented as average ± SD. (B) A collagen type I promoter construct (pH 20) was co-transfected with expression constructs for RFXB and/or RFXBSV into HASMCs as indicated. Cells were treated with IFN-γ and/or TGF-β for 24 h. Data were obtained and analyzed as described under (A). (C, D) HASMCs were infected with lentiviral stocks carrying an empty vector (pLenti6/V5), RFXB or RFXBSV as indicated. Cells were treated with IFN-γ and/or TGF-β 24 h after infection. RNA or protein was extracted 24 h after treatment. Message (C) and protein (D) levels of collagen type I were assessed by real-time PCR and Western blot, respectively. Data were processed and presented as described in Figure 1.