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Generation of RESC DNA from oligonucleotides. (a) Illustration of primer positions and PCR reactions required to generate a RESC DNA fragment in four rounds of PCR starting from 16 oligonucleotides. In each round, the two corresponding PCR products and outside primers from the previous round are used for amplification. Arrows indicate direction of elongation. (b) Agarose gel showing wRESC PCR products and their size obtained in four PCR rounds as illustrated in (a).
