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. 2009 May 29;37(13):e93. doi: 10.1093/nar/gkp450

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — sdc^RESC rescue in vitro and in vivo. (a) αSdc western blot from Drosophila S2 cells transiently transfected with pMT-Gal4, with (+) or without (–) UAS::Sdc^dsRNA, and UAS::sdc with wild-type (wt), or RESC sequence (Δ24, 24-mer changes; Δ12, 12-mer changes; Δ6, 6-mer changes). (b) αSdc western blot from adult flies ubiquitously expressing Sdc without (wt) or with (Δ6) RESC sequence, in the presence (+) or absence (–) of ubiquitous Sdc RNAi. (c) Quantification of unfused segments of tracheal dorsal branches per animal, in wild-type (sdc^+/+), sdc²³ homozygous (sdc^−/⁻) mutants, tracheal Sdc RNAi alone, or with tracheal rescue sdc^RESC. n ≥ 10; error bars = CI; *P < 0.01.