Table 1.
Primer sequences used for real-time PCR.
| Name | Accession no. | Primer sequence (5' to 3') | Product size (bp) | |
|---|---|---|---|---|
| MGF | X06108 | Sense | CGTCTTCACATCTCTTCTACC | 122 |
| Antisense | TGGTCCACACACGAACTG | |||
| Akt1 | NM_033230 | Sense | AGAAGGAGGTCATCGTTGC | 120 |
| Antisense | GGTCGTGGGTCTGGAATG | |||
| mTOR | NM_019906 | Sense | TCTTCTTCCAGCAAGTTCAGC | 97 |
| Antisense | GAATCAGACAGGCACGAAGG | |||
| S6K1 | NM_031985 | Sense | CGGGTACTTGGTAAAGGG | 150 |
| Antisense | CCGCTCTGCTTTTGTATG | |||
| Myogenin | NW_047395 | Sense | CAGTGAATGCAACTCCCACA | 85 |
| Antisense | CAAATGATCTCCTGGGTTGG | |||
| FoxO1 | AF247812 | Sense | CACCTTGCTATTCGTTTGC | 130 |
| Antisense | CTGTCCTGAAGTGTCTGC | |||
| Myostatin | AF019624 | Sense | GGCAGAGTATTGATGTGAAGAC | 119 |
| Antisense | TGGGAAGGTTACAGCAAGATC | |||
| MAFbx | NM_133521 | Sense | GCAAAACATAAGACTCATACG | 83 |
| Antisense | GTAGAGTGGTCTCCATTCG | |||
| MuRF1 | NM_080903 | Sense | AGGTGAAGGAGGAACTGAG | 86 |
| Antisense | AACTGCTCTCGGTACTGG | |||
| GAPDH | AF106860 | Sense | GGATGCAGGGATGATGTTCT | 116 |
| Antisense | AAGGGCTCATGACCACAGTC | |||
| β2M | NM_012512 | Sense | TCCACCCACCTCAGATAG | 149 |
| Antisense | AGCATATACATCGGTCTCG |