Figure 2.

Effect of the PI3K inhibitors on integrin-mediated eosinophil adhesion. (A) Effect of PI3K inhibition on IL-5–induced β₂-integrin–mediated adhesion to plated BSA. Eosinophils were preincubated with the specified concentrations of wortmannin (squares) or LY294002 (triangles) for 30 min, or dominant-negative TAT-Δp85 (circles) for 15 min at 37°C, followed by addition of IL-5 and then further incubated for 30 min at 37°C. The adherence of eosinophils was assessed in the presence of inhibitors as detailed in MATERIALS AND METHODS. Each point represents the mean ± SEM. *P < 0.05 and **P < 0.01 compared with each control, n = 5. (B) Effect of PI3K inhibition on β₁-integrin–mediated eosinophil adhesion to plated VCAM-1. Eosinophils were preincubated with the specified concentrations of wortmannin or LY294002 for 30 min, or dominant-negative TAT-Δp85 for 15 min at 37°C, and then were adhered to VCAM-1–coated wells for 30 min at 37°C. The adherence of eosinophils was measured as above (n = 5).