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The physical association between PI3K and PKC-δ. Eosinophils (5 × 106/group) were stimulated with 10 ng/ml of IL-5 or buffer for 10 min at 37°C, and the cells were lysed and immunoprecipitated by anti-p85 subunit of PI3K antibody. The immunioprecipitated proteins were separated by SDS-PAGE and were probed by anti–PKC-δ (left panel) or anti-p85 (right panel) antibody after stripping. The results shown are representative of four different experiments.
