Figure 6.

Effects of inhibition of PI3K or PKCδ on IL-5–induced ERK1/2 activation. Eosinophils were incubated with 100 nM wortmannin and 10 μM rottlerin for 20 min, or 600 nM TAT-Δp85 for 15 min at 37°C, followed by addition of 10 ng/ml IL-5 for 10 min at 37°C. Eosinophils were lysed, and the lysates were loaded on 10% SDS-PAGE, followed by immunoblotting with anti-phosphorylation–specific ERK1/2 Ab (top panel) or anti-ERK1/2 Ab (bottom panel). The result shown is representative of four different experiments.