Figure 3.

2DE gels containing airway epithelial cell proteins from rats exposed to ozone for 90 d followed by a single intraperitoneal injection of 50 mg/kg 1-NN are displayed above (Exposure Group 4). Three different pH ranges were used to achieve maximum sensitivity. The proteins were visualized with SYPRO Ruby protein stain and a Typhoon fluorescent scanner. The 14 protein spots determined to be adducted by 1-NN metabolites through phosphorimaging are circled and labeled with the respective protein ID number. Note that the abundance of spots number 12 and 13 are below the detection limit for SYPRO Ruby and, therefore, no spots are visible. The circles indicate the gel area that was excised for protein identification. No spots were found to be adducted in the basic region.