Figure 3.

Activation of other transcription factors in response to P. aeruginosa. (A–D) 1HAEo⁻ cells stimulated with P. aeruginosa PAO1 (10⁸ cfu) were lysed at timed intervals (min) and nuclear lysates screened for the presence of P-NF-κB, P-ATF-2, P-CREB, and P-C/EBP β. 1HAEo⁻ cells were transfected with a NF-κB, AP-1, CREB, or C/EBP luciferase reporter construct and either pretreated for 24 h with 100 nM cyclosporin (CsA) or untreated (Control). Following stimulation for 4 h with heat-killed P. aeruginosa PAO1 (10⁸ cfu), luciferase activity was measured and reported as fold activity compared with the unstimulated (Unstim) condition. *P < 0.05, **P < 0.001 compared with control for the PAO1 stimuli.