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. 2005 Apr 28;33(2):161–168. doi: 10.1165/rcmb.2004-0365OC

Figure 3.

Figure 3.

Inhibitors of ROS generation and transport decrease ROFA-AEC lung surfactant gelation. (A) A549 cells were pretreated for 15 min and then cotreated during exposure to soluble ROFA (100 μg/ml for 16 h) with ROS inhibitors, phytic acid (PA, 500 μM), deferoxamine (DF, 1 mM), or sodium benzoate (NaB, 50 mM) and the conditioned supernatants were evaluated by interfacial storage modulus measurements, Gs' (elasticity). Each inhibitor significantly decreased ROFA-AEC gelation of lung surfactant (*†‡P < 0.005, n = 3). (B) Transport of mitochondrially-generated ROS into the cytoplasm was inhibited by the mitochondrial anion channel blocker, DIDS (before and during exposure to soluble ROFA), and the conditioned supernatant evaluated. DIDS significantly inhibited ROFA-AEC surfactant gelation (*P < 0.005, n = 3). y-Axis is logarithmic scale.