Figure 2.

Effect of 24 h exposure of BEAS2B cells to TNF-α (100 ng/ml), IFN-γ (100 ng/ml), IL-4 (10 ng/ml), and control medium on cell surface expression of B7-H1 (upper left panel) and mRNA levels of B7-H1 (upper right panel, n = 5–9), B7-DC (lower left panel, n = 5), and B7-H3 (lower right panel, n = 3). Cells were stimulated as described in MATERIALS AND METHODS in the presence or absence of fluticasone (10⁻⁷ M), and then processed for flow cytometry or analysis by real-time PCR. Values shown are mean ± SEM fluorescence intensity or mean ± SEM fold change in mRNA from medium control. *P < 0.05 compared with control condition. ⁺P < 0.05 compared with no steroid. **P < 0.01 compared with control condition. ⁺⁺P < 0.01 compared with no steroid.